Assessment of Regression Methods for inference of regulatory networks involved in circadian regulation.

We assess the accuracy of three established regression methods for reconstructing gene and protein regulatory networks in the context of circadian regulation. Data are simulated from a recently published regulatory network of the circadian clock in Arabidopsis thaliana , in which protein and gene interactions are described by a Markov jump process based on Michaelis-Menten kinetics. We closely follow recent experimental protocols, including the entrainment of seedlings to different light-dark cycles and the knock-out of various key regulatory genes. Our study provides relative assessment scores for the comparison of state-of-the art regression methods, investigates the influence of systematically missing values related to unknown protein concentrations and mRNA transcription rates, and quantifies the dependence of the performance on the degree of recurrency.

Andrej Aderhold, Dirk Husmeier, V. Anne Smith, Andrew J. Millar, and Marco Grzegorczyk